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Frecuencias genotípicas y alélicas de apolipoproteina e (APOE) en una muestra de adultos en Bogotá

Genotypic and allelic frequencies of apolipoprotein e (APOE) in a sample of adults in bogotá




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Rubio Gómez, C., Gutiérrez Castañeda, L. D. ., Pedraza , O. L., Torres Tobar, L. ., Hernández Acevedo, G. ., Suárez Camacho, A. ., Lozano Ruiz, Ángela G., & Camacho-Bermúdez, I. N. . (2021). Frecuencias genotípicas y alélicas de apolipoproteina e (APOE) en una muestra de adultos en Bogotá. Revista Repertorio De Medicina Y Cirugía, 30(2), 134-141. https://doi.org/10.31260/RepertMedCir.01217372.1008

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Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-CompartirIgual 4.0.

Cladelis Rubio Gómez
    Luz Dary Gutiérrez Castañeda
      Olga Lucia Pedraza
        Lilian Torres Tobar
          Gualberto Hernández Acevedo
            Alfonso Suárez Camacho
              Ángela Gisselle Lozano Ruiz
                Isis Norella Camacho-Bermúdez

                  Introducción: la apolipoproteína E (APOE) es una glicoproteína implicada en el transporte de moléculas lipídicas. Se han descrito tres alelos del gen APOE: Ɛ2, Ɛ3 y Ɛ4. Varios estudios demuestran asociación de la isoforma APOE4 con Alzheimer de inicio tardío. Objetivos: determinar las frecuencias genotípicas y alélicas del gen APOE en una muestra de adultos en Bogotá. Materiales y métodos: estudio observacional descriptivo de corte transversal. A partir de una muestra de sangre periférica se extrajo ADN genómico y se realizó PCR-Tetraprimer para la determinación de los alelos de APOE. Resultados: se incluyeron 1.254 sujetos, 942 mujeres (75%) y 312 hombres (25%) con edades entre 40 y 100 años. El alelo más frecuente fue el Ɛ3 (85%), seguido por Ɛ4 (11%) y Ɛ2 (2%). De la población que manifestó tener ascendencia cundiboyacense, 567 sujetos (74.6%) presentaban el genotipo Ɛ3/Ɛ3, mientras que 156 (20.4%) el Ɛ3/Ɛ4, 23 (3%) el Ɛ2/Ɛ3, 11 (1.5%) el Ɛ4/Ɛ4, y 4 (0.5%) el Ɛ2/Ɛ4. Los individuos con genotipo Ɛ2/Ɛ2 manifestaron no conocer el dato de ascendencia. Conclusiones: las frecuencias alélicas y genotípicas de APOE varían según el origen étnico, sin embargo es posible la identificación de sujetos con el genotipo menos frecuente (Ɛ2/Ɛ2) al analizar muestras de mayor tamaño. En los reportes previos en el país no se ha descrito el genotipo Ɛ2/Ɛ2, el cual fue identificado en la presente muestra como el de menor proporción.

                   


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