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Screening for precancerous lesions: criteria for representativeness of oropharynx sample collection for liquid-based cytology
Tamizaje de lesiones precancerosas: criterios de representatividad de la toma de muestra en orofaringe para citología en base líquida
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Parra tamayo, L. alexandra, Baracaldo, . S. D. P., Vargas, . J., Sánchez De Guzmán, G., Vásconez, J. N., Ortiz , N. E., Lombana Amaya, L. J., & Morante Caicedo, C. (2025). Screening for precancerous lesions: criteria for representativeness of oropharynx sample collection for liquid-based cytology. Journal of Medicine and Surgery Repertoire. https://doi.org/10.31260/RepertMedCir.01217372.1692
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Liliana alexandra Parra tamayo
Roles:
Stela Del Pilar Baracaldo
Jimena Vargas
Roles:
Judith Natalia Vásconez
Natascha Enriqueta Ortiz
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SUMMARY
Introduction: Liquid-based cytology (LBC) has emerged as a promising technique for detecting precancerous lesions in the oropharynx. However, the lack of standardization in sample collection poses a significant challenge. Variability in collection methods, due to the absence of clear guidelines and differences in professional training, can impact the quality and reliability of results. Establishing uniform criteria for sample collection is essential to improve the accuracy and reliability of liquid cytology as a screening tool.
Methodology: A retrospective, observational, and descriptive study was conducted with the objective of describing 10 oral cavity and oropharyngeal samples without known HPV history, available in the pathology department. The criteria used for the evaluation of these samples were documented and analyzed, based on current literature. Since these samples are not routinely collected.
Conclusions: Both conventional and liquid-based exfoliative cytology are safe and reliable for detecting preinvasive lesions in the oral cavity and oropharynx. The Bethesda 2014 system's quality criteria, originally developed for cervical and anal cytology, are applicable to oral cytology due to similarities in cellularity and cytomorphological characteristics. Cytological preparations revealed superficial cells measuring 40-60 micrometers, with well-defined, irregular cytoplasmic borders and translucent cytoplasm with occasional keratinization. These findings indicate that liquid-based cytology provides an adequate representation of cellularity and morphology in the oropharynx, aligning with Bethesda system criteria for other areas
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