Production of agarose blocks for fungal visualization
Producción de bloques de agarosa para visualización de hongos
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Objective: to establish a protocol for obtaining fungal blocks using agarose as a matrix. Methods: the fungi were included in agarose, the process was standardized and the protocols were tested in microwave oven, conventional and in the automatic processor for obtaining sheets following the usual histological technique. Conclusions: the processor protocol to obtain multiple sheets is reproducible and the preparations allowed easy visualization of the fungi with H & E and Gomori colorations. Abbreviations: H & E, hematoxylin-eosin.
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