Findings in bronchoalveolar lavage processed using a cytospin technique and convential cytology
Hallazgos del lavado broncoalveolar procesado con técnica de cytospin y citología convencional
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Abstract
The cytological interpretation of bronchoalveolar lavage (BAL) specimen is currently performed using conventional cytology and cytospin preparations. The atter features concentrated preparations in a 6-mm area for sample visualisation and reduced reading time, with no deterioration in quality.
Objective: To determine the differences in reading time and quality, between the conventional technique and the cytospin technique.
Study design: A cross-sectional study was conducted on BAL specimens that were processed using the conventional technique and the cytospin technique. Reading time for diagnosis between the 2 techniques was compared. Quality was assessed taking into account, cell preservation and inflammatory cells in mears. The difference in time was calculated using the Wilcoxon test.
Results: Eighty (80) BAL slides were examined, 40 by the conventional technique and 40 by the cytospin technique. A differential count was not performed in 6 cases, in 4 due to excessive number of squamous cells and in 2 cases, processed by the conventional technique, due to exhibiting less than 300 cells. A marked degeneration of epithelial cells was observed using the conventional technique, showing a significant difference in reading time (P<.001).
Conclusions: A significant difference was found between reading times of the 2 techniques, which was longer when using the conventional technique. Better quality smears were observed when using the cytospin technique.
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2. Meyer KC. The clinical utility of bronchoalveolar lavage in interstitial lung disease - is it really useful? Expert Rev Respir Med. 2014;8:133–5.
3. García E. Lavado bronquioloalveolar. En: Rodríguez J, de Agustín D, editores. Cuadernos de citopatología. Aparato respiratorio-I (Técnicas. Células normales lavado bronquioloalveolar). Colombia: Diaz de Santos; 2003. p. 9–11.
4. Fariña González J, Rodríguez Costa J. Citología respiratoria y pleural. Madrid: Editorial Médica Panamericana; 1996. p. 194.
5. Gold WM, Murray JF. Atlas de técnicas en medicina respiratoria. Madrid: S.A. Elsevier Espan˜ a; 2003. p. 512.
6. Díaz J, García P, De la Barra R, Gasep J, Levican J, Quiroga T. Utilidad de la citocentrifugación en el diagnóstico bacteriológico microscópico de fluidos corporales. Rev Chil Infectol. 2002;19:167–73.
7. Solomides C, Johnston W, Elson C. Respiratory tract. En: Bibbo M, Wilbur D, editores. Comprehensive Cytopathology. Londres: Elsevier; 2008. p. 247–87.
8. Laportadez R, López García-Gallo C, Mora G, Trisán A. Indicaciones e interpretación del lavado broncoalveolar. Medicine. 2006;9:4343–5.
9. Chamberlain DW, Braude AC, Rebuck AS. A critical evaluation of bronchoalveolar lavage. Criteria for identifying unsatisfactory specimens. Acta Cytol. 1987;31: 599–605.
10. Baughman RP, Dohn MN, Loudon RG, Frame PT. Bronchoscopy with bronchoalveolar lavage in tuberculosis and fungal infections. Chest. 1991;99:92–7.
11. Allaouchiche B, Jaumain H, Dumontet C, Motin J. Early diagnosis of ventilator-associated pneumonia. Is it possible to define a cutoff value of infected cells in BAL fluid? Chest. 1996;110:1558–65.
12. Varón FA, Buitrago AF. Concordancia entre el gram y el cultivo del lavado broncoalveolar en pacientes con neumonía asociada al ventilador. [trabajo de grado]. Bogotá: Universidad del Rosario; 2010.
13. Levy H, Horak DA, Lewis MI. The value of bronchial washings and bronchoalveolar lavage in the diagnosis of lymphangitic carcinomatosis. Chest. 1988;94:1028–30.
14. Poletti V, Poletti G, Murer B, Saragoni L, Chilosi M. Bronchoalveolar lavage in malignancy. Semin Respir Crit Care Med. 2007;28:534–45.