Molecular techniques for the identification and typing of human papillomavirus
Técnicas moleculares para la identificación y tipificación del virus del papiloma humano
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Correa Vásquez, M. del P., López, N. N., & Rubiano Sechagua, J. R. (2005). Molecular techniques for the identification and typing of human papillomavirus. Journal of Medicine and Surgery Repertoire, 14(2), 69–73. https://doi.org/10.31260/RepertMedCir.v14.n2.2005.382
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Abstract
The use of molecular identification techniques such as those analyzed in this article (polymerase chain reaction, hybrid capture technique, southern dot and dot blot) allows to classify and typify the human papilloma virus present, to confirm the association of some cervical lesions with viral types of high and low grade, significantly orienting a more accurate diagnosis compared to other methods of detection or routine screening. However, the implementation of these techniques as a routine paraclinical method would be difficult for patients due to its high cost.
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References
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Clive C. Masure M. Bory JP et al. Hybrid Capture II based Huma Papillomavirus detection a sensitive test to detect toutine High — grade Cervical Lesions: A Preliminary study on 1518 women. Br J Cancer 1999; 80 (9):
Faro S D, Soper E. D. Enfermedades infecciosas en la mujer. México: Mc Grawn Hill; 2002 p 504-521.
Giannoudis A. Van Duin M, Snijders PJF et al. Variations in the E2-binding domain of HPV 16 are associated with highgrade sqamous intraepithelial lesions of the cervix. Br J Cancer 2001: 84 (8): 1058-1063.
Guerrero A, Avila C, Velasco P. Mejía Roberto F. ADN del HPV detectado en células escamosas atipicas de significado indeterminado ASCUS empleando la PCR: Reporte DE dos casos Act Cancer 2000; 30 (2).
Herrera H, Restrepo de Stiefken P. El virus del papiloma humano y su relación con la neoplasia cervical humana. Bogotá: Pontificia Universidad Javeriana, 2000.
Kuen W, Cheung K,N. Leslie, Li M S et al. Transitional Cell Metaplasia of the Uterine Cervix is Related to Human Papillomavirus: Molecular Analysis in Seven Patients with Cytohistologic Correlation. Can Cytop 2002; 96: 250-258.
Kulasingam L S, Hughes P J, Kiviat B N et al. Evaluation of Human Papillomavirus Testing in Primary Screening for Cervical Abnormalities: Comparison of Sensitivity, Specificity, and Frequency of Referral. JAMA 2002; 288 (14): 1749-57.
Liaw K, Hildesheim A, Burk D R et al. A Prospective Study of Human Papillomavirus (HPV) Type 16 DNA Detection by Polymerase Chain Reaction and its Association with Acquisition and Persistence of Other HPV Types. J Infect Dis 2001; 183: 8-15.
Luque J. Herraéz A. Texto ilustrado de biología molecular e ingeniería Genética, conceptos, técnicas y aplicaciones en ciencias de la salud. Madrid: Harcourt, 2001.
Matsura Y, Kawagoe T, Toki N, Sugihara K, Kashimura M. Low Grade Cervical intraepithelial Neoplasia Associated with Human Papillomavirus Infection. Act Cytol 1998; 42 (3): 625-630.
Molano M. Prevalence and determinante of HPV infection among Colombian women with normal cytology. Br J Cancer, 2002; 87: 324-333.
Moral R. Laboratorio y Atlas de Citología. Madrid Mac GrawHill, 1995. p 78-86.
Palo G, Chyanen W, Dexeus S. Patología y Tratamiento del Tracto Genital Inferior. Barcelona: Masson; 2000.
Picconi M A, Alonio V, Carranca García A et al. Variantes Moleculares del Virus del Papiloma Humano (HPV) Tipos 16 y 18 en Adenocarcinomas de Cerviz. Medicina de Buenos Aires 2000; 60: 889-894.
Sambrook J, Russeli D. Molecular Cloning a Laboratory Manual. Cold Spring Halberd, 2 nd ed. New York: Laboratory Press, 2001. v. 1
Satoshi K, Yasutaka K, et al. Detection of human papillomaviruses in cervical neoplasia using multiple sets of generic polymerase chain reaction primers. Gynecol Oncol 2001; 81: 47-52.
Serman F. Cáncer Cervicouterino; epidemiología, historia natural y rol del virus papiloma humano. Perspectivas en prevención y tratamiento. Rev Chil Obstet Ginecol 2002; 67 (4): 318-23.
Szuhai K, Sandhaus E, Kolkman-Ulijee M S et al. Novel strategy for Human Papillomavirus Detection and Genotyping with SybrGreen an Molecular Beacon Polymerase Chain Reaction. Am J Pathol 2001; 159 (5): 1651-1660.
Van Den Brule JC, Adriann P R, Fransens-Daalmeijer N et al. GP5+ / 6+ PCR followed by Reverse Line Blot Analysis Enables Rapid and Higt-Throughput identification of Human Papillomavirus Genotypes. J Clin Microbiol 2002; 40 (83): 779-87.
Vince A, Kutela N, ISCIC-BES J et al. Clinical utility of molecular detection of papillomavirus in cervical samples by capture technology. J Clin Virol 2002; 25: 109-12.
Zhang A, Maner S, Betz R et al. Genetic Alterations in Cervical Carcinomas: Frequent Low-level amplifications of Oncogenes are Associated with Human Papillomavirus Infection. Int J Cancer 2002; 101: 427-33.
Canseco A L. Detección molecular del VPHs y otros virus genitales en muestras de tejido cervical. Chiapas: Universidad Antonio de Chiapas. 2000. Tesis para obtener el título de Químico Fármaco biólogo.
Clive C. Masure M. Bory JP et al. Hybrid Capture II based Huma Papillomavirus detection a sensitive test to detect toutine High — grade Cervical Lesions: A Preliminary study on 1518 women. Br J Cancer 1999; 80 (9):
Faro S D, Soper E. D. Enfermedades infecciosas en la mujer. México: Mc Grawn Hill; 2002 p 504-521.
Giannoudis A. Van Duin M, Snijders PJF et al. Variations in the E2-binding domain of HPV 16 are associated with highgrade sqamous intraepithelial lesions of the cervix. Br J Cancer 2001: 84 (8): 1058-1063.
Guerrero A, Avila C, Velasco P. Mejía Roberto F. ADN del HPV detectado en células escamosas atipicas de significado indeterminado ASCUS empleando la PCR: Reporte DE dos casos Act Cancer 2000; 30 (2).
Herrera H, Restrepo de Stiefken P. El virus del papiloma humano y su relación con la neoplasia cervical humana. Bogotá: Pontificia Universidad Javeriana, 2000.
Kuen W, Cheung K,N. Leslie, Li M S et al. Transitional Cell Metaplasia of the Uterine Cervix is Related to Human Papillomavirus: Molecular Analysis in Seven Patients with Cytohistologic Correlation. Can Cytop 2002; 96: 250-258.
Kulasingam L S, Hughes P J, Kiviat B N et al. Evaluation of Human Papillomavirus Testing in Primary Screening for Cervical Abnormalities: Comparison of Sensitivity, Specificity, and Frequency of Referral. JAMA 2002; 288 (14): 1749-57.
Liaw K, Hildesheim A, Burk D R et al. A Prospective Study of Human Papillomavirus (HPV) Type 16 DNA Detection by Polymerase Chain Reaction and its Association with Acquisition and Persistence of Other HPV Types. J Infect Dis 2001; 183: 8-15.
Luque J. Herraéz A. Texto ilustrado de biología molecular e ingeniería Genética, conceptos, técnicas y aplicaciones en ciencias de la salud. Madrid: Harcourt, 2001.
Matsura Y, Kawagoe T, Toki N, Sugihara K, Kashimura M. Low Grade Cervical intraepithelial Neoplasia Associated with Human Papillomavirus Infection. Act Cytol 1998; 42 (3): 625-630.
Molano M. Prevalence and determinante of HPV infection among Colombian women with normal cytology. Br J Cancer, 2002; 87: 324-333.
Moral R. Laboratorio y Atlas de Citología. Madrid Mac GrawHill, 1995. p 78-86.
Palo G, Chyanen W, Dexeus S. Patología y Tratamiento del Tracto Genital Inferior. Barcelona: Masson; 2000.
Picconi M A, Alonio V, Carranca García A et al. Variantes Moleculares del Virus del Papiloma Humano (HPV) Tipos 16 y 18 en Adenocarcinomas de Cerviz. Medicina de Buenos Aires 2000; 60: 889-894.
Sambrook J, Russeli D. Molecular Cloning a Laboratory Manual. Cold Spring Halberd, 2 nd ed. New York: Laboratory Press, 2001. v. 1
Satoshi K, Yasutaka K, et al. Detection of human papillomaviruses in cervical neoplasia using multiple sets of generic polymerase chain reaction primers. Gynecol Oncol 2001; 81: 47-52.
Serman F. Cáncer Cervicouterino; epidemiología, historia natural y rol del virus papiloma humano. Perspectivas en prevención y tratamiento. Rev Chil Obstet Ginecol 2002; 67 (4): 318-23.
Szuhai K, Sandhaus E, Kolkman-Ulijee M S et al. Novel strategy for Human Papillomavirus Detection and Genotyping with SybrGreen an Molecular Beacon Polymerase Chain Reaction. Am J Pathol 2001; 159 (5): 1651-1660.
Van Den Brule JC, Adriann P R, Fransens-Daalmeijer N et al. GP5+ / 6+ PCR followed by Reverse Line Blot Analysis Enables Rapid and Higt-Throughput identification of Human Papillomavirus Genotypes. J Clin Microbiol 2002; 40 (83): 779-87.
Vince A, Kutela N, ISCIC-BES J et al. Clinical utility of molecular detection of papillomavirus in cervical samples by capture technology. J Clin Virol 2002; 25: 109-12.
Zhang A, Maner S, Betz R et al. Genetic Alterations in Cervical Carcinomas: Frequent Low-level amplifications of Oncogenes are Associated with Human Papillomavirus Infection. Int J Cancer 2002; 101: 427-33.