Frequency of DNA-HPV-H in atypical squamous cells. Case series: ASC-US and LSIL
Frecuencia de DNA-HPV-H en células escamosas atípicas. Serie de casos: ASC-US y LSIL
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Puerto de Amaya, M. B., Olaya Contreras, M., Rey Cediel, K., Diaz Jiménez, K., & Pérez M., C. H. (2014). Frequency of DNA-HPV-H in atypical squamous cells. Case series: ASC-US and LSIL. Journal of Medicine and Surgery Repertoire, 23(2), 121–126. https://doi.org/10.31260/RepertMedCir.v23.n2.2014.726
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Abstract
Objective: to determine the polymerase chain reaction (PCR) infection by high-risk human papilloma virus (DNA-HPV-H) and the presence of morphological change with atypia in the cytology of women working in a hospital and an educational entity. Methods: series of cases of cervical uterine samples with conventional and liquid-based cytologies, and PCR for DNA-HPV-H; Patients who had one or more positive results in cytology (ASC-US and LSIL) were included. Results: DNA-HPV-H typing was positive in 12 of 41 cases. A greater number of cytologies with cellular atypia were observed in the range of young women (22-49 years), compared with the largest ones (50-72 years). The positive cases for DNA-HPV-H in both conventional and liquid-based cytology were similar. There were 11 with simple infection and one multiple positive for high risk (HPV-H and HPV-16). Conclusions: CRP identified 12 patients infected with HPV at high risk, 11 with simple and multiple infections; The group that predominated was HPV-H (9 cases), followed by HPV-18 subtype (5) and HPV-16 (1). Abbreviations: ASC-US indeterminate atypia of squamous cells; ASC-H, atypia that does not rule out high grade; HPV, human papillomavirus; LSIL, low-grade squamous intraepithelial lesion; HSIL, high-grade squamous intraepithelial lesion.
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References
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25. Munger K. The molecular biology of cervical cancer. J Cell Biochem Suppl. 1995;23: 55-60.
26. McKee GT. Cytopathology. London; Baltimore: Mosby-Wolfe; 1997.
27. Colombia. Ministerio de la Protección Social. Recomendaciones para la tamizacion de neoplasias de cuello uterino en mujeres sin antecedentes de patologia cervical (Preinvasora o Invasora) en Colombia: guia practica clinica numero 3 [monografía en Internet]. ]. Bogotá: Instituto Nacional de Cancerología; 2007 [citado 29 abr 2014]. Disponible en: http://www.cancer.gov.co/documentos/RecomendacionesyGuias/GuiaN3.pdf
28. Colombia. Ministerio de la Protección Social. Recomendaciones para la tamizacion de neoplasias de cuello uterino en mujeres sin antecedentes de patologia cervical (Preinvasora o Invasora) en Colombia: guia practica clinica numero 3 [monografía en Internet]. ]. Bogotá: Instituto Nacional de Cancerología; 2007 [citado 29 abr 2014]. Disponible en: http://www.cancer.gov.co/documentos/RecomendacionesyGuias/GuiaN3.pdf
2. Kjaer SK, van den Brule AJ, Paull G, Svare EI, Sherman ME, Thomsen BL, et al. Type specific persistence of high risk human papillomavirus (HPV) as indicator of high grade cervical squamous intraepithelial lesions in young women: population based prospective follow up study. BMJ. 2002 Sep 14; 325(7364):572.
3. Walboomers JM, Jacobs MV, Manos MM, Bosch FX, Kummer JA, Shah KV, et al. Human papillomavirus is a necessary cause of invasive cervical cancer worldwide. J Pathol. 1999;189(1):12-9.
4. Grillo-Ardila CF, Martínez-Velásquez MY, Morales-López B. Virus del papiloma humano: aspectos moleculares y cáncer de cérvix. Rev Colomb Obstet Ginecol. 2008;59:310-5.
5. Ramzy I. Clinical cytopathology & aspiration biopsy : fundamental principles and practice. Norwalk, Conn.: Appleton & Lange; 1990.
6. zur Hausen H. Papillomaviruses and cancer: from basic studies to clinical application. Nat Rev Cancer. 2002;2(5):342-50.
7. Solomon D, Nayar R. El Sistema Bethesda para informar la citología cervical: definiciones, criterios y notas aclaratorias. Buenos Aires: Journal; 2005.
8. Lie AK, Risberg B, Borge B, Sandstad B, Delabie J, Rimala R, et al. DNA- versus RNA-based methods for human papillomavirus detection in cervical neoplasia. Gynecol Oncol. 2005 Jun;97(3):908-15.
9. Beldi MC, Tacla M, Caiaffa-Filho H, Ab’saber A, Siqueira S, Baracat EC, et al. Implementing human papillomavirus testing in a public health hospital: challenges and opportunities. Acta Cytol.. 2012;56(2):160-5.
10. Wright TC Jr, Stoler MH, Sharma A, Zhang G, Behrens C, Wright TL. Evaluation of HPV-16 and HPV-18 genotyping for the triage of women with high-risk HPV+ cytology-negative results. Am J Clin Pathol. 2011 Oct; 136(4):578-86.
11. Saez de Santamaria J, Agustin Vazquez D. Cuadernos de Citopatologia: Citología líquida. Madrid : Díaz de Santos, c2006
12. Campo Rodríguez P, Puerto de Amaya M. Comparación entre las técnicas de citologia compartida: convencional vs bases liquida. Repert med y cir. 2011;20:240-4.
13. De La Fuente-Villarreal D, Guzmán López S, Barboza-Quintana O, González Ramírez RA. Biologia del Virus del Papiloma Humano y tecnicas del diagnostico. Med Univer. 2010;12(49):231-8.
14. Cotran RS, Kumar V, Collins T, Robbins SL. Patología estructural y funcional [de] Robbins. Madrid, España: McGraw-Hill/Interamericana; 2000.
15. Wright TC Jr, Massad LS, Dunton CJ, Spitzer M, Wilkinson EJ, Solomon D. 2006 consensus guidelines for the management of women with cervical intraepithelial neoplasia or adenocarcinoma in situ. Am J Obstet Gynecol. 2007 Oct; 197(4):340-5.
16. Saslow D, Solomon D, Lawson HW, Killackey M, Kulasingam SL, Cain J, Garcia FA, et al. American Cancer Society, American Society for Colposcopy and Cervical Pathology, and American Society for Clinical Pathology screening guidelines for the prevention and early detection of cervical cancer. CA Cancer J Clin. 2012 May-Jun; 62(3):147-72.
17. Spinillo A, Dal Bello B, Gardella B, Roccio M, Dacco MD, Silini EM. Multiple human papillomavirus infection and high grade cervical intraepithelial neoplasia among women with cytological diagnosis of atypical squamous cells of undetermined significance or low grade squamous intraepithelial lesions. Gynecol Oncol. 2009;113(1):115-9.
18. Bibbo M. Comprehensive cytopathology. Philadelphia: Saunders; 1997.
19. Wright T.C KR, Ferenczy A. Precancerous Lesions of the cervix. In: Kurman, Robert J., Hedrick Ellenson, Lora, Ronnett, Brigitte M, editors. Blaustein`s pathology of the female genital tract. New York: Springer; 2006. p. 253-324.
20. Manos MM, Kinney WK, Hurley LB, Sherman ME, Shieh-Ngai J, Kurman RJ, et al. Identifying women with cervical neoplasia: Using human papillomavirus dna testing for equivocal papanicolaou results. JAMA. 1999;281(17):1605-10.
21. Farag R, Redline R, Abdul-Karim FW. Value of combining HPV-DNA testing with follow-up Papanicolaou smear in patients with prior atypical squamous cells of undetermined significance. Acta cytol. 2008;52(3):294-6.
22. Dunne EF, Unger ER, Sternberg M, McQuillan G, Swan DC, Patel SS, et al. Prevalence of hpv infection among females in the united states. JAMA. 2007;297(8):813-9.
23. OMS. Control Integral del Cancer Cevicouterino: guía de practicas esenciales. Ginebra: OMS; 2011.
24. Jing Shu Y GE. comprehensive Cancer Cytopathology of the Cervix Uteri Correlation with histopathology. New York: McGraw-Hill;1995.
25. Munger K. The molecular biology of cervical cancer. J Cell Biochem Suppl. 1995;23: 55-60.
26. McKee GT. Cytopathology. London; Baltimore: Mosby-Wolfe; 1997.
27. Colombia. Ministerio de la Protección Social. Recomendaciones para la tamizacion de neoplasias de cuello uterino en mujeres sin antecedentes de patologia cervical (Preinvasora o Invasora) en Colombia: guia practica clinica numero 3 [monografía en Internet]. ]. Bogotá: Instituto Nacional de Cancerología; 2007 [citado 29 abr 2014]. Disponible en: http://www.cancer.gov.co/documentos/RecomendacionesyGuias/GuiaN3.pdf
28. Colombia. Ministerio de la Protección Social. Recomendaciones para la tamizacion de neoplasias de cuello uterino en mujeres sin antecedentes de patologia cervical (Preinvasora o Invasora) en Colombia: guia practica clinica numero 3 [monografía en Internet]. ]. Bogotá: Instituto Nacional de Cancerología; 2007 [citado 29 abr 2014]. Disponible en: http://www.cancer.gov.co/documentos/RecomendacionesyGuias/GuiaN3.pdf